Congress shall make no law respecting an establishment of religion, or prohibiting the free exercise thereof; or abridging the freedom of speech or of the press; or the right of the people peaceably to assemble, and to petition the Government for a redress of grievances.
Offered by Rev. Mr. Duché, in Carpenter's Hall, Philadelphia, 7 September 1774:
Be Thou Present, O God of Wisdom, and direct the council of this Honorable Assembly; enable them to settle all things on the best and surest foundations; that the scenes of blood may be speedily closed; that Order, Harmony and Peace may be effectually restored, and that Truth and Justice, Religion and Piety, prevail and flourish among the people.
Preserve the health of their bodies, and the vigor of their minds, shower down on them, and the millions they here represent, such temporal Blessings as Thou seest expedient for them in this world, and crown them with everlasting Glory in the world to come. All this we ask in the name and through the merits of Jesus Christ, Thy Son and our Saviour, Amen.
Congress recorded their appreciation for this prayer:
Wednesday, September 7, 1774, 9 o'clock a.m. Agreeable to the resolve of yesterday, the meeting was opened with prayers by the Rev. Mr. Duché. Voted, That the thanks of Congress be given to Mr. Duché... for performing divine Service, and for the excellent prayer, which he composed and delivered on the occasion.
Reprinted in America's God and Country, page 221.
For a discussion of the phrase "separation of church and state" try this post.